John Bowne was an English emigrant who arrived in in New Netherland, or Vlissingen (now Flushing) in 1649. He fought against Governor Pieter Stuyvesant's edict to restrict religious freedom by allowing Quakers to meet in his home. Bowne was arrested, fined and imprisoned for months by Gov. Stuyvesant and even deported due to his religious activities, though he was later set free by the Directors of the West India Company. He returned to his home later and acquired more land, including that designated for the Flushing Quaker Meeting House and a burial ground, where he was buried upon his death in 1695.

Flushing had the previous name of Flushing Creeke by the original inhabitants who lived there, the Matinecock people, part of the larger Algonquin nation. While the Matinecock people are said to have sold land to the Dutch, and possibly to Bowne as well, there was also documented violence against them prior to this, as well as a smallpox epidemic that devastated the community years later in 1652. Members of the Matinecock tribe remain in Queens today.